Mcp subfamily composed of at least four members mcp 1, 2, 3, and 4. Human mcp1 ccl2 precoated elisa kit catalog number. Highly sensitive 96well immunoassay kit with additional reagents. Cytokines and chemokines and related molecules, energy metabolism and body weight regulation. Page 1 of 1 mouse ccl2 mcp1 uncoated elisa catalog number. Antigen detection cholera, smallpox, anthrax, elisa protocol influenza, and stds step 3. Mcp1 chemoattractant protein involed in monocyte migration and infiltration monocyte chemotractant protein1 mcp1, also known as ccl2, is a key chemokine involved in the. As of march 6, 2018, human mcp 1 elisa kit has been redeveloped. Standards and samples are added to the wells, and mcp1 binds to the immobilized capture antibody.
The kit is stable for a period of up to 3 months from the date of receipt. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. Product name rat mcp 1 elisa kit catalog number elrmcp1 kit components component size description mcp 1 microplate item a 96 wells 12 strips x 8 wells coated with antirat mcp 1. The human mcp1 ccl2 elisa kit accurately measures natural human mcp1 ccl2 levels quantified versus standard curves obtained and is. Rat mcp1 elisa development kit leinco technologies. The omnikine murine je mcp 1 elisa kit contains the components necessary for quanititative determination of natureal or recombinant murine je mcp 1 concentrations within any experimental sample including cell lysates, serum and plasma. The rat mcp1 elisa enzymelinked immunosorbent assay kit is an in vitro enzymelinked immunosorbent assay for the quantitative measurement of rat mcp1 in cell lysate and tissue lysate. Mcp subfamily composed of at least four members mcp1, 2, 3, and 4. Mcp1 elisa kit mouse monocyte chemoattractant protein 1. Immunogen recombinant rat mcp1 application for research use only. Rat mcp1 elisa development kit contains the key components required for the quantitative measurement of natural andor recombinant mcp1 in a sandwich elisa format. Raybio human mcp1 ccl2 elisa kit for cell culture supernatants, plasma, and serum samples. Dilute the antigen to 12 ugml in coating solution 2. Immunoreagents is the direct us manufacturer of quality antibodies and reagents used in research and in vitro diagnostics.
Wash buffer concentrate 20x item b 25 ml of 20x concentrated solution. Mcp1 elisa kit human monocyte chemoattractant protein 1. Rat mcp 1 elisa development kit contains the key components required for the quantitative measurement of natural andor recombinant mcp 1 in a sandwich elisa format. Your affordable premium quality elisa kits provider. It is a member of the ccbeta family of cytokines, characterized by the cysc.
The below protocol is a sample protocol for mouse mcp1 ccl2 elisa kit using a biotinylated detection antibody and streptavidinhrp. The enzymelinked immunosorbent assay elisa is a frequently used application which determines the concentration of an analyte in solution. Rat mcp1 elisa development kit contains the key components required for the quantitative measurement of natural andor recombinant rat mcp1 in a sandwich elisa format within the range of 162000 pgml. As of march 6, 2018, human mcp1 elisa kit has been redeveloped. A human mcp 1 specific mouse monoclonal antibody is first coated on a 96well plate. Mcp 1 is mainly expressed by macrophages in response to a wide range of cytokines such as il6, tnfalpha and il1beta, but can, upon stimulation, also be produced by a variety of cells and tissues, such as fibroblasts, endothelial cells or certain tumor cells.
Add 100 ul of diluted antigen to appropriate wells. Not for further distribution without written consent. Boster is proud to announce the newest picokine fast elisa kits. Mouse mcp1 elisa kit picokine sandwich high sensitivity elisa kit for quantitative detection of mouse mcp1. Mcp 1 chemoattractant protein involed in monocyte migration and infiltration monocyte chemotractant protein 1 mcp 1, also known as ccl2, is a key chemokine involved in the migration of monocytes and macrophages to sites of active inflammation.
Mcp 1was characterized as a monocytespecific chemoattractant that was later shown to attract also t lymphocytes and nk cells. Preparation of mcp1 standard dilutions general elisa protocol 1. Urine and serum samples were centrifuged at 2500 r. Mcp1was characterized as a monocytespecific chemoattractant that was later shown to attract also t lymphocytes and nk cells. The human monocyte chemoattractant protein 1 ccl2 mcp 1 uncoated elisa kit contains prematched antibody pairs, plates and reagents for performing quantitative enzyme linked immunosorbent assays elisa to detect and quantify protein levels of human mcp 1.
Kassay kamiya biomedical companya biomedical company rev. Elisa inter assay consistency is measured using wells from different plates from the same batch productionlot. Prepare all reagents and working standards as directed in the previous sections. Please read the datasheet in its entirety before using this product. The sandwich elisa protocol provided here is representative of most readytouse elisa kits for measurement of cytokines, chemokines, growth factors, and other extracellular targets. Mouse mcp1 elisa max standard elisa development systems engineered for sensitive, precise and reliable serum and plasma cytokine quantitationbiolegends elisa max sets. Apr 01, 2010 enzymelinked immunosorbent assay elisa mcp1 levels in eps and in cell culture supernatants were measured by a human ccl2 mcp1 elisa readysetgo kit ebioscience, san diego, ca. Using the elisa protocol described below, this kit provides sufficient reagents to assay mcp 1 in approximately 1500 elisa plate wells. Mcp1 is believed to be identical to je, a gene whose expression is induced in mouse fibroblasts by plateletderived growth factor cochran and others 1983. Bgk500 elisa kit for the accurate quantitation of human mcp1 ccl2 concentrations in cell culture supernates. Invitrogen mcp1ccl2 mouse uncoated elisa kit with plates specifically engineered for accurate and precise measurement of mouse ccl2 protein levels from samples including serum, plasma, and cell culture supernatants. Preparation of mcp 1 standard dilutions general elisa protocol 1. Enzymelinked immunosorbent assay elisa mcp1 levels in eps and in cell culture supernatants were measured by a human ccl2 mcp1 elisa readysetgo kit.
A 384well simplestep elisa microplate is available to use as an alternative to the 96well microplate provided with simplestep elisa kits. Mcp1 monocyte chemotactic protein1, ccl2 has a predicted molecular weight of approximately 11kda, however, it may run at a higher molecular weight due to glycosylation. Human mcp1monocyte chemotactic protein 1 elisa kittk06435. Product name rat mcp1 elisa kit catalog number elrmcp1 kit components component size description mcp1 microplate item a 96 wells 12 strips x 8 wells coated with antirat mcp. Human ccl2 mcp1 uncoated elisa enzymelinked immunosorbent assay for quantitative detection of human ccl2 mcp1 catalog number 887399 pub. A human mcp1 specific mouse monoclonal antibody is first coated on a 96well plate. We measured random samples of human mcp1 ccl2 elisa kit picokine within the same batchlot to ensure the consistency of the kits performances. Human mcp1 ccl2 elisa kit ab179886 is a singlewash 90 min sandwich elisa designed for the quantitative measurement of mcp1 protein in human serum, plasma, urine, and. Biolegends elisa max deluxe set is a sandwich enzymelinked immunosorbent assay elisa.
Mcp1 elisa kit mouse monocyte chemoattractant protein. Human mcp1monocyte chemotactic protein 1 elisa kit. Invitrogen mcp1ccl2 mouse uncoated elisa kit with plates. This mcp1ccl2 human elisa kit from aviscera bioscience enables to detect mcp1 on human samples. Elisa technical guide and protocols thermo fisher scientific. The loss rate of human mcp1monocyte chemotactic protein 1 elisa kit is less than 5% within the expiration date under appropriate storage condition. Mcp 1 is produced by a variety of cell types, either constitutively or after induction by oxidative stress, cytokines, or growth factors. Rat mcp1 elisa development kit contains the key components required for the quantitative measurement of natural andor recombinant rat mcp1 in a sandwich elisa format within the.
Mcp 1 monocyte chemotactic protein 1, ccl2 has a predicted molecular weight of approximately 11kda, however, it may run at a higher molecular weight due to glycosylation. Prepare all reagents, samples and standards as instructed in the manual. A human mcp1 specific mouse monoclonal antibody is first coated on a 96well. Depending on the protein of interest, antibodies, buffers, or substrates being used, this general protocol may need to be optimized. Mouse mcp1 ccl2 elisa kit from elisa genie is a precoated immunoassay with a sensitivity of 9. Kt020 product the kassay human mcp1 elisa is an enzymelinked immunosorbent. We have identified new recombinant monoclonal antibodies to provide improved performance and consistency. The following is a protocol that was developed for the tgf. This immunoassay has been shown to accurately quantitate recombinant human mcp 1. With automated equipment the assay is easily scalable for set up in highthroughput and can handle a large number of samples in parallel, making it a popular. Invitrogen mcp1ccl2 mouse uncoated elisa kit with plates 10. Rat mcp1 ccl2 elisa kit for cell and tissue lysates. The mouse monocyte chemoattractant protein 1 ccl2 mcp 1 uncoated elisa kit contains prematched antibody pairs, plates and reagents for performing quantitative enzyme linked immunosorbent assays elisa to detect and quantify protein levels of mouse mcp 1. Mcp 1 is a member of the cytokine family and plays an important role in immunoregulation and inflammatory processes.
The rat mcp1 elisa enzymelinked immunosorbent assay kit is an in vitro enzymelinked immunosorbent assay for the quantitative measurement of rat mcp1 in cell lysate and tissue. The mouse monocyte chemoattractant protein1ccl2 mcp1 uncoated elisa kit contains prematched antibody pairs, plates and reagents for performing quantitative. Biotin mouse antihuman mcp1 can be paired with purified mouse antihuman mcp1 clone 10f7. Sek104 to achieve the best assay results, this manual must be read carefully before using this product and the assay is run as. Mcp1 is produced by a variety of cell types, either. Human mcp1 ccl2 elisa kit from elisa genie is a precoated immunoassay with a sensitivity of 9. The human ccl2 mcp1 uncoated elisa reagent set with or without highaffinity binding microwell plates contains the necessary reagents, buffers and diluents for performing quantitative enzyme linked immunosorbent assays elisa. Incubate 2 hours at room temperature or 4 c overnight. Standards and samples are added to the wells, and mcp 1 binds to the immobilized capture antibody. Mcp1 is mainly expressed by macrophages in response to a. Mcp1 elisa kit human monocyte chemoattractant protein. Mcp1 measurements were performed as per the manufacturers recommendations. The monocyte chemoattractant protein1 mcp 1ccl2 is a member of the cc chemokine family, and a potent chemotactic factor for monocytes. Sets provide all the timesavings, convenience and performance of a highend, commercial elisa kit for a fraction of the price.
The biotin mouse antihuman mcp1 should be titrated 0. Monocyte chemotactic protein1 mcp1ccl2 is associated. Kt020 product the kassay human mcp1 elisa is an enzymelinked immunosorbent assay for the quantitative determination of mcp1 in human cell culture supernatant, serum, plasma and amniotic fluid. Determine the number of microwell strips required to test the desired number of samples. This elisa reagent set is specifically engineered for accurate and precise measurement of human. Human mcp1 is composed of 76 amino acids and is kda in size.
Classroom exchange hiv, sars and west nile antigen of simulated bodily fluids viruses, common cold, step 2. Protocol i elisa for tracking disease outbreaks step 1. Human mcp 1 is composed of 76 amino acids and is kda in size. Aspirate and wash 7 times with 30 sec to 1 min soaks. Sek104 to achieve the best assay results, this manual must be read carefully before using this product and the assay is run as summarized in the general elisa protocol. The human ccl2 mcp1 uncoated elisa reagent set with or without highaffinity binding microwell plates contains the necessary reagents, buffers and diluents for performing. Mcp1ccl2 human uncoated elisa kit with plates invitrogen. The enzyme linked immunosorbent assay elisa is a powerful method for detecting and quantifying a specific protein in a complex mixture. Notes mcp 1 ccl2 is a chemokine expressed by fibroblasts, macrophages, endothelial cells, mast cells, osteoblasts, ameloblasts, epithelial cells, and astrocytes after stimulation with inflammatory. The omnikine murine jemcp1 elisa kit contains the components necessary for quanititative determination of natureal or recombinant murine jemcp1 concentrations within any. Track disease transmission protocol ii antigen detection elisa pregnancy, drug, gmo, antigen. Notes mcp1 ccl2 is a chemokine expressed by fibroblasts, macrophages, endothelial cells, mast cells, osteoblasts, ameloblasts, epithelial cells, and astrocytes after stimulation with inflammatory stimuli. Originally described by engvall and perlmann 1971, the method enables analysis of protein samples immobilized in microplate wells using specific antibodies.
Bgk500 elisa kit for the accurate quantitation of human mcp1 ccl2 concentrations in cell culture supernates, serum, plasma, and other biological fluids. Sandwich elisas allow for the detection and quantification of an analyte in a sample by using known analyte concentrations as standards and plotting absorbance of known concentrations vs known standard. Mcp1ccl2 mouse uncoated elisa kit with plates invitrogen. Jemcp1 murine omnikine elisa kit assaybiotechnology. Elisa intra assay consistency is measured using wells from the same plateassay kit.